激光共聚焦显微镜(带活细胞培养系统) LSM 980 Confocal Microscope -1 (with cell incubator)

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设备型号

LSM 980

当前状态

管理员

刘健威 Jianwei LIU,李思仪 Rita Siyi LI

放置地点

E4-123B
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名称

激光共聚焦显微镜(带活细胞培养系统) LSM 980 Confocal Microscope -1 (with cell incubator)

资产编号

BC-MS-0001

型号

LSM 980

规格

产地

厂家

所属品牌

Zeiss

出产日期

购买日期

所属单位

生物科学中央实验室 Biosciences Central Research Facility (GZ)

使用性质

科研

所属分类

BioCRF-影像平台 Imaging Core

资产负责人

阚光嫣 Guangyan KAN

联系电话

联系邮箱

jianweiliu@hkust-gz.edu.cn

放置地点

E4-123B
  • 主要规格&技术指标
  • 主要功能及特色
  • 设备使用相关说明
  • 备注
主要规格&技术指标
1. Objectives: 2.5X/ 0.085; 5X/ 0.16; 10X/ 0.45; 20X/ 0.8; 63X oil/ 1.4 calibrated for Airy Scan;
2. Lasers: 405nm, 30mW; 445nm, 30mW; 488nm, 30mW; 514nm, 30mW; 561nm, 25mW; 594nm, 8mW; 639nm, 25mW;
3. Fluorescence Unit:LED light source: UV, 385nm; Blue, 475nm; Green, 555nm; Red, 630nm;
4. Detectors:
2x PMTs; 1x Confocal GaAsP; 1x Airyscan GaAsP; 1x T-PMT;
5. Incubation System:
*Temperature adjustable between 28 - 60°C (Accuracy +/- 0.1°C);
*CO2 concentration adjustable between 0 – 8% (Accuracy +/- 0.1%);
*Humidity control;
*Individual Cell culture system, suitable for 35 mm and 60 mm dishes;
6. Scanner Performance:
*Max. FPS @ 512x512, max resolution X/Y/Z nm:
*Confocal: 13 FPS, 200/200/500;
*Airyscan SR: 4.7 FPS, 120/120/350 or 90/90/270(jDCV);
*Multiplex SR-4Y: 25 FPS, 140/140/450;
*Multiplex SR-8Y: 47.5 FPS, 120/160/450;
*Multiplex CO-8Y: 34.4 FPS, 180/200/450;
7. Sample Insert:
1x Slide; 1x Petri Dish 35 mm; Universal Mounting Frame;
8. Software:
ZEN 3.5 Software
Including AI Sample Finder, ZEN Connect and Experiment Designer, FRAP, FRET, Macro, Tiles and position.
主要功能及特色
应用领域:
1. 活体样品的超高分辨率共聚焦成像
2. 生物学成像,例如Ca2 +成像
3. 大面积组织切片成像
4. 三维成像
5. 时间序列成像
6. 多色光谱成像。

Applications:
1. Super resolution confocal imaging of live samples, photoactivation, or photoconversion in live cells or organisms.
2. Capturing fast events such as Ca2+ imaging.
3. Large-area tissue section imaging.
4. Z-stack imaging.
5. Time series imaging.
6. Multispectral imaging.
设备使用相关说明
User Declaration for Instrument Usage
仪器使用声明
We (I) hereby declare that the confocal microscopy instrument will be solely used for study biology. Samples will be cells of bio-organism, such as Zebrafish, Drosophila melanogaster, or mice, or even palnts. We use the microsope to obatin clear confocal fluoresecence images with high quality, obtain clear and sharp multilayer z-plane structure (opotical slices), time series imagers, large field mosaic, etc.
我们(我)在此声明,共聚焦显微镜仪器将仅用于生物学研究。样本将是生物体的细胞,如斑马鱼、果蝇、小鼠,甚至是植物。我们使用显微镜获得高质量的清晰共聚焦荧光图像,获取清晰锐利的多层z平面结构(光学切片)、时间序列图像、大视野拼图等。
We (I) certify that the microsope and any replica thereof (in case of technology also goods derived from derived goods of the items) will not be used, in their entirety or in part, in connection with
我们(我)证明该显微镜及其任何复制品(在技术转让的情况下,也包括由技术衍生出的物品)将不会全部或部分地用于如下用途:
the development, production, handling, operation, maintenance, storage, detection, identification or dissemination of chemical, biological, radiological or nuclear weapons / other explosive devices or the development, production, maintenance or storage of missiles / other systems capable of delivering such weapons, or the development, production, handling, operation, maintenance or storage of cluster munitions or anti-personnel mines or human rights violations.
研发、生产、操作、运行、维护、存储、探测、识别或者传播化学武器、生化武器、放射性武器或核武器或其他爆炸装置;或研发、生产、维护、存储导弹或其他可用于运输上述武器的系统;或研发、生产、操作、运行、维护或存储集束弹药或人员杀伤地雷;或侵犯人权。
The microsope and any replica thereof (in case of technology also goods derived from derived goods of the items) will be used for civil purposes only.
显微镜及其任何复制品(在技术转让的情况下,也包括由技术衍生出的物品)将仅用于民用用途。

凡涉及安全风险的样品(如生物危害、危险化学品、人体来源样品、纳米材料、放射性材料等)须通过生物科学中央实验室准入审查;未通过审查不得入内。相关合规义务及由此产生的责任由本人及所属机构自行承担。
Any samples involving safety risks (such as biohazards, hazardous chemicals, human-derived samples, nanomaterials, or radioactive materials) must pass the BioCRF's access review; samples that do not pass the review may not be brought into the BioCRF. Compliance obligations and any resulting liabilities shall be borne by me and my affiliated institution.
备注
Please clean up the table after doing experiments.
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