MALDI TOF/TOF Mass Spectrometer（rapifleX）
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3/人使用者
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54/次机时次数
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2126/小时总时长
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12/次送样次数
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5/人收藏者
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收费标准
机时0元/小时送样详见检测项目 -
设备型号
rapifleX -
当前状态
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管理员
文美玲 Wendy Meiling WEN 020-88338595 -
放置地点
E4-126/133走廊-132A
- 仪器信息
- 检测项目
- 附件下载
- 公告
- 同类仪器
名称
MALDI TOF/TOF Mass Spectrometer（rapifleX）
资产编号
BC-PM-0016
型号
rapifleX
规格
产地
厂家
所属品牌
Bruker
出产日期
购买日期
所属单位
生物科学中央实验室 Biosciences Central Research Facility (GZ)
使用性质
科研
所属分类
BioCRF-蛋白质&代谢组学平台 Proteomics & Metabolomics Core
资产负责人
文美玲 Wendy Meiling WEN
联系电话
020-88338595
联系邮箱
meilingwen@hkust-gzedu.cn
放置地点
E4-126/133走廊-132A
- 主要规格&技术指标
- 主要功能及特色
- 样本检测注意事项
- 备注
主要规格&技术指标
Ion source: MALDI ion source
Laser type: BRUKER scanning smartbeam™ 3D laser, 355 nm wavelength
Laser frequence: 0.001kHz-10 kHz
Number of laser shots: >1*1010 (10 billion)
Mass Resolution：Linear mode ≥ 1,200 for Cytochrome C (m/z 12,361) FWHM； Reflect mode ≥ 50,000 for Somatostatin 28 (m/z 3,147.5) FWHM; MS/MS Fragment Resolution，≥ 1,500 for [Glu1]-Fib fragment m/z 175, ≥ 5,000 for [Glu1]-Fib fragment m/z 684, ≥ 7,500 for [Glu1]-Fib fragment m/z 1,056, ≥ 10,000 for [Glu1]-Fib fragment m/z 1,441
MS Sensitivity：Linear mode, 500 fmol BSA (m/z 66,000) at S/N ≥ 100:1 shown with 1,000 laser shots；Reflect mode , 250 amol [Glu1]-Fibrinopeptide B (m/z 1,570.7) at S/N ≥ 200:1 shown with 2,000 laser shots; MS/MS mode: 250 amol [Glu1]-Fibrinopeptide B fragment 1,056 Da at S/N ≥ 30:1 shown with 1,000 laser shots
Linear mode MS Accuracy: (protein mixture)≤ 60 ppm with external calibrant, ≤ 50 ppm with internal calibrant
Reflect mode MS Accuracy: (peptide mixture)≤ 5 ppm with external calibration, ≤ 1.0 ppm with internal calibration
MS/MS Mass Accuracy: ≤ 0.025 Da for relevant fragments of [Glu1]-Fibrinopeptide B (m/z 1,570)
Imaging Pixel shape and size: Squared pixel for best tissue coverage with variable pixel size of 20 µm to 100 µm
Imaging Speed: Up to 40 pixel / sec
Laser type: BRUKER scanning smartbeam™ 3D laser, 355 nm wavelength
Laser frequence: 0.001kHz-10 kHz
Number of laser shots: >1*1010 (10 billion)
Mass Resolution：Linear mode ≥ 1,200 for Cytochrome C (m/z 12,361) FWHM； Reflect mode ≥ 50,000 for Somatostatin 28 (m/z 3,147.5) FWHM; MS/MS Fragment Resolution，≥ 1,500 for [Glu1]-Fib fragment m/z 175, ≥ 5,000 for [Glu1]-Fib fragment m/z 684, ≥ 7,500 for [Glu1]-Fib fragment m/z 1,056, ≥ 10,000 for [Glu1]-Fib fragment m/z 1,441
MS Sensitivity：Linear mode, 500 fmol BSA (m/z 66,000) at S/N ≥ 100:1 shown with 1,000 laser shots；Reflect mode , 250 amol [Glu1]-Fibrinopeptide B (m/z 1,570.7) at S/N ≥ 200:1 shown with 2,000 laser shots; MS/MS mode: 250 amol [Glu1]-Fibrinopeptide B fragment 1,056 Da at S/N ≥ 30:1 shown with 1,000 laser shots
Linear mode MS Accuracy: (protein mixture)≤ 60 ppm with external calibrant, ≤ 50 ppm with internal calibrant
Reflect mode MS Accuracy: (peptide mixture)≤ 5 ppm with external calibration, ≤ 1.0 ppm with internal calibration
MS/MS Mass Accuracy: ≤ 0.025 Da for relevant fragments of [Glu1]-Fibrinopeptide B (m/z 1,570)
Imaging Pixel shape and size: Squared pixel for best tissue coverage with variable pixel size of 20 µm to 100 µm
Imaging Speed: Up to 40 pixel / sec
主要功能及特色
The rapifleX® combines state-of-the-art technology with advanced informatics into a system that gives you speed, robustness, versatility and confidence. The platform offers a complete characterization system with unprecedented speed of analysis and performance，such as: protein characterization, biomarker discovery, polymer analysis, high throughput screening, Glycopeptide analysis and tissue imaging, and so on.
Speed - highest throughput provides 5-20 times increase in speed
Robustness - User removable ion source for easy cleaning, designed for maximum uptime in 24/7 operations
Confidence
Ion optics adapt to application
No compromise, maximum resolution for full molecular weight range
Maximized sensitivity for MS and MS/MS analysis
MS/MS mass range for large peptides such as glycopeptides, di/trisulfide peptides and difficult non-tryptic peptides
Superior resolution for high selectivity in complex mixtures
Supreme precursor ion selection for confidence in MS/MS data quality
High dynamic range
True pixels – the smartbeam 3D laser features a mode which leads to true squared pixels, harnessing more tissue and ultimately increasing sensitivity.
Speed - highest throughput provides 5-20 times increase in speed
Robustness - User removable ion source for easy cleaning, designed for maximum uptime in 24/7 operations
Confidence
Ion optics adapt to application
No compromise, maximum resolution for full molecular weight range
Maximized sensitivity for MS and MS/MS analysis
MS/MS mass range for large peptides such as glycopeptides, di/trisulfide peptides and difficult non-tryptic peptides
Superior resolution for high selectivity in complex mixtures
Supreme precursor ion selection for confidence in MS/MS data quality
High dynamic range
True pixels – the smartbeam 3D laser features a mode which leads to true squared pixels, harnessing more tissue and ultimately increasing sensitivity.
样本检测注意事项
1. Molecular Mass Determination
2. Peptide mass fingerprinting (PMF)
3. MALDI Imaging
2. Peptide mass fingerprinting (PMF)
3. MALDI Imaging
备注
已更新送样表格，送样时请下载。
The sample submission form has been updated. Please download it when submitting samples.
The sample submission form has been updated. Please download it when submitting samples.
检测项目
附件下载
公告
同类仪器
