1. Absorbance:
*Plate types: 6- to 384-well plates;
*Wavelength selection: Double monochromators;
*Wavelength range: 200–1,000 nm;
*Light source: Xenon flash lamp;
*Read-out range: 0–6 Abs;
*Linear measurement range: 0–4 Abs (96-well plate) at 450 nm, ±2%;
0–3 Abs (384-well plate) at 450 nm, ±2%;
*Accuracy: 0.003 Abs or ±2%, at 200–399 nm (0–2 Abs);
0.003 Abs or ±1%, at 400–1,000 nm (0–3 Abs);
*Precision: Standard deviation (SD) <0.001 Abs or coefficient of variation (CV) <0.5%, at 450 nm (0–3 Abs);
2. Fluorescence intensity:
*Plate types: 6- to 1,536-well plates;
*Wavelength selection: Double excitation and emission monochromators;
*Excitation wavelength range: 200–1,000 nm;
*Emission wavelength range: 270–840 nm;
*Light source: Xenon flash lamp;
*Sensitivity: Top reading: <0.4 fmol fluorescein/well (black 384-well plate)
Bottom reading: <4 fmol fluorescein/well (clear bottom, black 384-square well plate);
*Dynamic range: Top reading: >6 decades
Bottom reading: >5.5 decades;
3. Time-resolved fluorescence:
*Plate types: 6- to 1,536-well plates;
*Wavelength selection: Filters (spectral scanning with double excitation and emission monochromators);
*Excitation wavelength range: Fixed to 334 nm (spectral scanning 200–840 nm);
*Emission wavelength range: 400–700 nm (spectral scanning 270–840 nm);
*Light source: Xenon flash lamp;
*Sensitivity: <1 amol Eu/well (white, low-volume 384-well plate);
*Dynamic range: >6 decades;
4. Luminescence:
*Plate types: 6- to 1,536-well plates (spectral scanning from 6- to 384-well plates);
*Wavelength selection: Direct or filters (spectral scanning with double monochromators);
*Wavelength range: 360–670 nm;
*Sensitivity: <7 amol ATP/well (white 384-well plate);
*Dynamic range: >7 decades;
5. AlphaScreen:
*Plate types: 6- to 1,536-well plates;
*Wavelength selection: Filters;
*Excitation wavelength range: Fixed to 680 nm;
*Emission wavelength range: 400–660 nm;
*Light source: LED;
*Sensitivity: <100 amol phosphotyrosine/well (white 384-well plate);
6. Dispensing:
*Plate types: 6- to 384-well plates;
*No. of dispensers: None, one, or two;
*Syringe size: 1 mL (standard), 5 mL (optional);
*Dispensing volume: 2–5,000 µL, in 1 µL increments (1 mL syringe)
5–25,000 μL, in 5 µL increments (5 mL syringe);
*Accuracy: <1 μL with 50 μL (0.4 mm tip), <0.2 μL with 5 μL (0.25 mm tip);
*Precision: <1 μL with 50 μL (0.4 mm tip), <0.25 μL with 5 μL (0.25 mm tip);
*Dead volume: Reagent loss <100 μL, total tubing volume <800 μL;
7. Incubator and shaker:
*Temperature range: From ambient +4°C to 45°C;
*Shaking type: Orbital;
8. Integrated gas module:
*CO2 concentration range: 0.1–15%;
*CO2 concentration stability: ±0.3% at 5% CO2;
*O2 concentration range: 1–21%;
*O2 concentration stability: ±0.3% at 1% O2;
9. General features:
*Measurement modes: Endpoint, kinetic, spectra, multipoint and kinetic spectra;
*Measurement speed: Reads a 96-well plate in 15 sec, a 384-well plate in 45 sec, and a 1,536-well plate in 135 sec (minimum times);
10. μDrop:
*Applications: measurements of DNA, RNA, protein samples, or spectral scanning;
*Measurement range: dsDNA detection range from a few micrograms to a few milligrams per milliliter;
*Sample capacity and volume: 32 (µDrop Duo Plate) sample positions for quick and easy measurement of sample volumes down to 2 μL;
*Feature: Compatible with an eight-channel pipette for sample addition;
11. Sofeware: With instrument control functions and advanced analytical functions;