Agilent 5200 片段分析仪 Agilent 5200 Fragment Analyzer
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5/人使用者
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91/次机时次数
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698/小时总时长
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31/次送样次数
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20/人收藏者
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收费标准
机时0元/小时送样详见检测项目 -
设备型号
Agilent 5200 Fragment Analyzer -
当前状态
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管理员
郭超 Chao GUO 020-88338593 -
放置地点
E4-126/133走廊-130
- 仪器信息
- 预约资源
- 检测项目
- 附件下载
- 公告
- 同类仪器
名称
Agilent 5200 片段分析仪 Agilent 5200 Fragment Analyzer
资产编号
BC-GM-0003
型号
Agilent 5200 Fragment Analyzer
规格
产地
厂家
Agilent
所属品牌
Agilent
出产日期
购买日期
所属单位
生物科学中央实验室 Biosciences Central Research Facility (GZ)
使用性质
科研
所属分类
BioCRF-基因组学平台 Genomics Core
资产负责人
郭超 Chao GUO
联系电话
020-88338593
联系邮箱
chaoguo@hkust-gz.edu.cn
放置地点
E4-126/133走廊-130
- 主要规格&技术指标
- 主要功能及特色
- 样本检测注意事项
主要规格&技术指标
1、标准SBS格式96孔板用于样品加载，可以同时注入和检测12个样品。
2、3个96样品取样托盘，可自动完成288个样品的连续取样分析。
3、毛细管电泳分离技术采用12通道33cm毛细管。300bp以内大小的片段的分辨率为3bp。
4、标准分析时间：60分钟内完成12个样品的分析检测（小于3kbp样品）
5、检测限：5 pg/µL（单组分样品）。
6、样品要求：PCR产物、基因组DNA、RNA等。样品不需要纯化、脱盐等后处理，可以直接注射分析。
7、可检测DNA范围：1～60000bp。可以对基因组DNA进行质量控制测试。
8、片段大小准确度：优于5%。
9、光源：LED光源，激发波长470nm。
10、检测器：高灵敏度CCD检测器，检测样品发射波长范围为500-600nm。
11、两种不同类型的分离凝胶可以同时放置，两种不同的应用可以连续进行，而无需手动干预，例如DNA和RNA的连续定性和定量分析。
1. standard SBS format 96-well plate for samples loading, 12 samples can be injected and detected simultaneously.
2. 96-sample sampling tray of 3, which can realize continuous sampling analysis of 288 samples under unattended operation.
3. Capillary electrophoresis separation technology with 12-channel 33cm capillary. The fragment size resolution within 300bp is 3bp.
4. Standard analysis time: complete the analysis and detection of 12 samples within 60 minutes (less than 3kbp samples)
5. Detection limit: 5 pg/µL (single-component sample).
6. Sample requirements: PCR products, genomic DNA, RNA, etc. The sample does not need post-processing such as purification and desalination, and can be directly injected for analysis.
7. Detectable DNA range: 1~60000bp. Quality control testing can be performed on genomic DNA.
8. Fragment size accuracy: better than 5%.
9. Light source: long-life LED light source with excitation wavelength of 470nm.
10. Detector: high-sensitivity CCD detector and the emission wavelength range of the detection sample is 500-600nm.
11. Two different types of separation gels can be placed at the same time, and the two different types of applications can be carried out continuously without manual intervention, such as continuous qualitative and quantitative analysis of DNA and RNA.
2、3个96样品取样托盘，可自动完成288个样品的连续取样分析。
3、毛细管电泳分离技术采用12通道33cm毛细管。300bp以内大小的片段的分辨率为3bp。
4、标准分析时间：60分钟内完成12个样品的分析检测（小于3kbp样品）
5、检测限：5 pg/µL（单组分样品）。
6、样品要求：PCR产物、基因组DNA、RNA等。样品不需要纯化、脱盐等后处理，可以直接注射分析。
7、可检测DNA范围：1～60000bp。可以对基因组DNA进行质量控制测试。
8、片段大小准确度：优于5%。
9、光源：LED光源，激发波长470nm。
10、检测器：高灵敏度CCD检测器，检测样品发射波长范围为500-600nm。
11、两种不同类型的分离凝胶可以同时放置，两种不同的应用可以连续进行，而无需手动干预，例如DNA和RNA的连续定性和定量分析。
1. standard SBS format 96-well plate for samples loading, 12 samples can be injected and detected simultaneously.
2. 96-sample sampling tray of 3, which can realize continuous sampling analysis of 288 samples under unattended operation.
3. Capillary electrophoresis separation technology with 12-channel 33cm capillary. The fragment size resolution within 300bp is 3bp.
4. Standard analysis time: complete the analysis and detection of 12 samples within 60 minutes (less than 3kbp samples)
5. Detection limit: 5 pg/µL (single-component sample).
6. Sample requirements: PCR products, genomic DNA, RNA, etc. The sample does not need post-processing such as purification and desalination, and can be directly injected for analysis.
7. Detectable DNA range: 1~60000bp. Quality control testing can be performed on genomic DNA.
8. Fragment size accuracy: better than 5%.
9. Light source: long-life LED light source with excitation wavelength of 470nm.
10. Detector: high-sensitivity CCD detector and the emission wavelength range of the detection sample is 500-600nm.
11. Two different types of separation gels can be placed at the same time, and the two different types of applications can be carried out continuously without manual intervention, such as continuous qualitative and quantitative analysis of DNA and RNA.
主要功能及特色
安捷伦5200生物分析仪系统是一种平行毛细管电泳仪器，可在15分钟内平行分离12个样本，从而提高实验效率。它对多种样品进行DNA质控和RNA质控（包括gDNA、小RNA、cfDNA、大片段DNA和总RNA）。该系统可以分离的样本类型的多样性使其成为进行个性化工作流程（如NGS库质控和CRISPR工作流程）的理想选择。
The Agilent 5200 Fragment Analyzer System is a parallel capillary electrophoresis instrument that increases experimental efficiency, separating 12 samples in parallel in 15 minutes. It performs DNA QC and RNA QC on a wide variety of samples including gDNA, small RNA, cfDNA, large fragment DNA, and total RNA. The diversity of sample types that the system can separate makes it ideal for personalized workflows, including NGS library QC and CRISPR workflows.
The Agilent 5200 Fragment Analyzer System is a parallel capillary electrophoresis instrument that increases experimental efficiency, separating 12 samples in parallel in 15 minutes. It performs DNA QC and RNA QC on a wide variety of samples including gDNA, small RNA, cfDNA, large fragment DNA, and total RNA. The diversity of sample types that the system can separate makes it ideal for personalized workflows, including NGS library QC and CRISPR workflows.
样本检测注意事项
RNA Assay (15nt):For the quantitative and qualitative analysis of total RNA from 200 to 6000 nt. Sample Volume:2ul. Qualitative Range: Total RNA: 5 - 500 ng/µL; mRNA: 1 - 100 ng/µL. Quantitative Range:Total RNA: 25 - 500 ng/µL; mRNA: 20 - 100 ng/µL
HS RNA Assay (15nt):For the quantitative and qualitative analysis of total RNA from 200 to 6000 nt. Sample Volume:2ul. Quantitative Range:Total RNA: 50 - 5000 pg/µL; mRNA: 500 - 5000 pg/µL
HS NGS Fragment Kit (1-6000bp):For the quantitative and qualitative analysis of NGS libraries and intermediates from 100 to 6000 bp at low concentrations. Sample Volume:2ul. Sizeing Range: 100 bp – 6,000 bp. Quantitative Range: Fragment:5 - 500 pg/µL; Smear:50 – 5,000 pg/µL
NGS Fragment Kit (1-6000bp):For the quantitative and qualitative analysis of NGS libraries and intermediates from 100 to 6000 bp.Sample Volume:2ul. Sizeing Range: 100 bp – 6,000 bp. Fragment Sensitivity Range:0.1 - 10 ng/µL. Smear Sensitivity Range:5 - 100 ng/µL
HS RNA Assay (15nt):For the quantitative and qualitative analysis of total RNA from 200 to 6000 nt. Sample Volume:2ul. Quantitative Range:Total RNA: 50 - 5000 pg/µL; mRNA: 500 - 5000 pg/µL
HS NGS Fragment Kit (1-6000bp):For the quantitative and qualitative analysis of NGS libraries and intermediates from 100 to 6000 bp at low concentrations. Sample Volume:2ul. Sizeing Range: 100 bp – 6,000 bp. Quantitative Range: Fragment:5 - 500 pg/µL; Smear:50 – 5,000 pg/µL
NGS Fragment Kit (1-6000bp):For the quantitative and qualitative analysis of NGS libraries and intermediates from 100 to 6000 bp.Sample Volume:2ul. Sizeing Range: 100 bp – 6,000 bp. Fragment Sensitivity Range:0.1 - 10 ng/µL. Smear Sensitivity Range:5 - 100 ng/µL
预约资源
检测项目
附件下载
公告
同类仪器
