LC-MS system（timsTOF HT MS-nanoElute 2 & Elute）
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2/人使用者
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17/次总次数
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440/小时总时长
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5/人收藏者
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收费标准
机时0元/小时送样详见检测项目 -
设备型号
timsTOF HT-nanoElute 2 & Elute -
当前状态
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管理员
文美玲 Wendy Meiling WEN 020-88338595 -
放置地点
E4-126/133走廊-132A
- 仪器信息
- 检测项目
- 附件下载
- 公告
- 同类仪器
名称
LC-MS system（timsTOF HT MS-nanoElute 2 & Elute）
资产编号
BC-PM-0012
型号
timsTOF HT-nanoElute 2 & Elute
规格
产地
厂家
所属品牌
Bruker
出产日期
购买日期
所属单位
生物科学中央实验室 Biosciences Central Research Facility (GZ)
使用性质
科研
所属分类
BioCRF-蛋白质&代谢组学平台 Proteomics & Metabolomics Core
资产负责人
文美玲 Wendy Meiling WEN
联系电话
020-88338595
联系邮箱
放置地点
E4-126/133走廊-132A
- 主要规格&技术指标
- 主要功能及特色
- 样本检测注意事项
- 设备使用相关说明
- 备注
主要规格&技术指标
nanoElute:
Sample loop: 20 µL
Sample loading range：1-10 µL（Partial loop）or 20 µL (full loop)
Sample tray: 2 sample plate (54 position/plate) or 96-well plate or 384-well plate
Sampler Temperature range: 15℃ below room temperature
Single-acting syringe pump
Flow： 50-2000 nL/min
Pressure of Loading pump: < 1000 bar
Column compartment temperature: 25-60℃
Elute:
High-pressure mixing binary pump：max. 1300 bar
Flow: 1-5000 µL/min
Mixer volume: 35 µL
Sample loading mode: full-loop/partial-loop/µL-pickup
Sample tray: 1 sample plate (108 position for 2mL-vial or 30 position for 10mL-vial) or 96-well plate or 384-well plate
Sampler Temperature range: 4-40℃
Column compartment temperature：15-90℃
timsTOF HT:
Scan rate: MS ≥ 50 Hz; Up to 300 Hz in dda-PASEF
MS range: up to 20000 m/z
Resolution: 60000 (1222 m/z)
Mass accuracy: Internal ≤ 0.8 ppm (tuning mix m/z 1221.990637, 30 spectral)；External ≤ 2 ppm (tuning mix m/z 1221.990637, 10 spectral)
Sensitivity: 1pg/µL Reserpine (m/z 609), S/N 100:1
Features: Trapped Ion Mobility Spectrometry (TIMS) is an IMS separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization. A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF.
Sample loop: 20 µL
Sample loading range：1-10 µL（Partial loop）or 20 µL (full loop)
Sample tray: 2 sample plate (54 position/plate) or 96-well plate or 384-well plate
Sampler Temperature range: 15℃ below room temperature
Single-acting syringe pump
Flow： 50-2000 nL/min
Pressure of Loading pump: < 1000 bar
Column compartment temperature: 25-60℃
Elute:
High-pressure mixing binary pump：max. 1300 bar
Flow: 1-5000 µL/min
Mixer volume: 35 µL
Sample loading mode: full-loop/partial-loop/µL-pickup
Sample tray: 1 sample plate (108 position for 2mL-vial or 30 position for 10mL-vial) or 96-well plate or 384-well plate
Sampler Temperature range: 4-40℃
Column compartment temperature：15-90℃
timsTOF HT:
Scan rate: MS ≥ 50 Hz; Up to 300 Hz in dda-PASEF
MS range: up to 20000 m/z
Resolution: 60000 (1222 m/z)
Mass accuracy: Internal ≤ 0.8 ppm (tuning mix m/z 1221.990637, 30 spectral)；External ≤ 2 ppm (tuning mix m/z 1221.990637, 10 spectral)
Sensitivity: 1pg/µL Reserpine (m/z 609), S/N 100:1
Features: Trapped Ion Mobility Spectrometry (TIMS) is an IMS separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization. A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF.
主要功能及特色
Built on the core principles of its predecessor, the nanoElute 2 has been refined for unrivalled robustness, ultimate chromatographic performance, and ease-of-use. The nanoElute 2 covers a broad range of applications from single-cell to high-throughput routine proteomics. This next generation system provides you with the highest chromatographic resolution resulting in exceptional peptide identification and quantification.
Elute LC systems are built on an innovative pump design that provides a virtually pulse free, precise, volumetric flow control which is independent of flow rates, solvent compressibility or composition.
All Elute LC systems are configured with two pairs of serial-coupled, individually controlled linear drive pump heads. This principle makes it easy to transfer ultra high performance liquid chromatography methods from one system to another, whether to an adjacent laboratory or across the globe.
The flow path is optimized by minimizing the gradient delay volume to deliver high chromatographic resolution, fast gradients and ultimate short cycle times, increasing sample throughput and reducing cost.
The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF® acquisition modes including PASEF®, dia-PASEF® and prm-PASEF® achieving flexibility in 4D-Proteomics™. Simply uncompromised proteomic depth at scale. The timsTOF platform stands out for its exceptional reliability and minimal maintenance needs, making it ideally suited for seamless integration into everyday laboratory workflows with maximum uptime and fast turnarounds. More than >7000 protein groups can be identified on average in only 5-minute gradient time with ease and across sites. Achieve top edge reproducibility over time without manual interference.
Elute LC systems are built on an innovative pump design that provides a virtually pulse free, precise, volumetric flow control which is independent of flow rates, solvent compressibility or composition.
All Elute LC systems are configured with two pairs of serial-coupled, individually controlled linear drive pump heads. This principle makes it easy to transfer ultra high performance liquid chromatography methods from one system to another, whether to an adjacent laboratory or across the globe.
The flow path is optimized by minimizing the gradient delay volume to deliver high chromatographic resolution, fast gradients and ultimate short cycle times, increasing sample throughput and reducing cost.
The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF® acquisition modes including PASEF®, dia-PASEF® and prm-PASEF® achieving flexibility in 4D-Proteomics™. Simply uncompromised proteomic depth at scale. The timsTOF platform stands out for its exceptional reliability and minimal maintenance needs, making it ideally suited for seamless integration into everyday laboratory workflows with maximum uptime and fast turnarounds. More than >7000 protein groups can be identified on average in only 5-minute gradient time with ease and across sites. Achieve top edge reproducibility over time without manual interference.
样本检测注意事项
1. 蛋白组学
若需蛋白前处理服务，具体方式请咨询仪器管理员；
若送肽段样品，请用户自行脱盐定量后送样；
不提供肽段标记服务，若用户需做标记蛋白组学，须自行标记肽段后再送样；
蛋白组学样本信息收集表详见附件”Proteomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
2. 小分子直接MS进样
需提供样品纯度证明，如：NMR谱图，LC-UV色谱图或者低分辨质谱图；
样本信息收集表详见附件”Small Molecule MS_Sample Submission Form“，送样时填写并交给仪器管理员；
3. 代谢组学
不提供样品前处理服务，用户需自行处理样品，并制备QC样品；
样本信息收集表详见附件”Metabolomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
4. 每周一和周三接收样品（E4-122）；
5. 如有不明确的其他事宜，请联系仪器管理员。
若需蛋白前处理服务，具体方式请咨询仪器管理员；
若送肽段样品，请用户自行脱盐定量后送样；
不提供肽段标记服务，若用户需做标记蛋白组学，须自行标记肽段后再送样；
蛋白组学样本信息收集表详见附件”Proteomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
2. 小分子直接MS进样
需提供样品纯度证明，如：NMR谱图，LC-UV色谱图或者低分辨质谱图；
样本信息收集表详见附件”Small Molecule MS_Sample Submission Form“，送样时填写并交给仪器管理员；
3. 代谢组学
不提供样品前处理服务，用户需自行处理样品，并制备QC样品；
样本信息收集表详见附件”Metabolomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
4. 每周一和周三接收样品（E4-122）；
5. 如有不明确的其他事宜，请联系仪器管理员。
设备使用相关说明
蛋白组学分析默认是1小时梯度，延长梯度加收20元/小时。
备注
1. Shotgun Protein Identification
• In-solution tryptic digestion service available
• Database search using PEAKS Studio (DDA & direct-DIA)
2. Protein Quantification
• Label-free Quantification（DDA or direct-DIA）
3. Direct infusion MS analysis for high purity sample (small molecule)
4. Untargeted Metabolomics Analysis (single mode)
• In-solution tryptic digestion service available
• Database search using PEAKS Studio (DDA & direct-DIA)
2. Protein Quantification
• Label-free Quantification（DDA or direct-DIA）
3. Direct infusion MS analysis for high purity sample (small molecule)
4. Untargeted Metabolomics Analysis (single mode)
检测项目
附件下载
公告
同类仪器
