悬尾实验（大小鼠）Tail suspension test (TST)
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收费标准
机时0元/小时 -
设备型号
SA210 -
当前状态
-
管理员
李存正 Cunzheng LI 020-88335662 -
放置地点
E4-4F LAF DEF侧入口-432A一更-432B二更-E区433A-1行为学E1433A-2行为学E2
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名称
悬尾实验（大小鼠）Tail suspension test (TST)
资产编号
LAF202501036
型号
SA210
规格
产地
中国
厂家
赛昂斯
所属品牌
赛昂斯
出产日期
2024-12-22
购买日期
2024-12-22
所属单位
实验动物中央实验室 Laboratory Animal Facility (GZ)
使用性质
科研
所属分类
LAF-行为测试 Behavioural test devices
资产负责人
李存正 Cunzheng LI
联系电话
020-88335662
联系邮箱
放置地点
E4-4F LAF DEF侧入口-432A一更-432B二更-E区433A-1行为学E1433A-2行为学E2
- 主要规格&技术指标
- 主要功能及特色
主要规格&技术指标
硬件外形尺寸：600x430x550mm，背板带 LED 发光
Tail Suspension Test SOP:
1. Pre-Test Preparation: Ensure that all equipment is operational before the experiment, including the video camera, suspension apparatus, data acquisition system, and software. Keep ambient lighting consistent throughout the session. Transport animals to the testing room in their home cages and allow at least 30 minutes of acclimation in a quiet environment to minimize stress-induced variability.
2. Apparatus Cleaning: Before each trial, wipe down the suspension hook, panel, and surrounding area with 75% ethanol to eliminate any residual odors or biological traces. Allow surfaces to air dry fully before introducing the next subject.
3. Camera and Setup Configuration: Mount the camera facing the testing panel, ensuring the entire body of the mouse will be visible during suspension. Connect the camera to the recording system using a video capture card. Adjust camera focus and position to avoid blind spots or motion artifacts in the video feed.
4. Software Setup: If behavior is recorded and analyzed using software, create a new experiment and define the testing zone within the camera’s view. Calibrate the video feed to match the physical dimensions of the apparatus. Set the trial duration to 6 minutes, and define behavioral parameters as required for analysis.
5. Behavioral Testing Procedure: Suspend the mouse by the tail using adhesive tape, securing it approximately 1–2 cm from the tip. Affix the tape to the suspension hook so that the mouse is freely hanging and cannot touch any surfaces or climb its tail. Begin video recording immediately after suspension. Observe the animal for 6 minutes, during which periods of immobility and struggling will be recorded manually or automatically, depending on the setup.
6. Post-Session Cleaning and Data Management: After the session, gently remove the tape and return the animal to its home cage. Clean the suspension area and hook again with 75% ethanol. Save and organize all video files and tracking data. Ensure proper labeling of experimental groups for subsequent analysis.
Tail Suspension Test SOP:
1. Pre-Test Preparation: Ensure that all equipment is operational before the experiment, including the video camera, suspension apparatus, data acquisition system, and software. Keep ambient lighting consistent throughout the session. Transport animals to the testing room in their home cages and allow at least 30 minutes of acclimation in a quiet environment to minimize stress-induced variability.
2. Apparatus Cleaning: Before each trial, wipe down the suspension hook, panel, and surrounding area with 75% ethanol to eliminate any residual odors or biological traces. Allow surfaces to air dry fully before introducing the next subject.
3. Camera and Setup Configuration: Mount the camera facing the testing panel, ensuring the entire body of the mouse will be visible during suspension. Connect the camera to the recording system using a video capture card. Adjust camera focus and position to avoid blind spots or motion artifacts in the video feed.
4. Software Setup: If behavior is recorded and analyzed using software, create a new experiment and define the testing zone within the camera’s view. Calibrate the video feed to match the physical dimensions of the apparatus. Set the trial duration to 6 minutes, and define behavioral parameters as required for analysis.
5. Behavioral Testing Procedure: Suspend the mouse by the tail using adhesive tape, securing it approximately 1–2 cm from the tip. Affix the tape to the suspension hook so that the mouse is freely hanging and cannot touch any surfaces or climb its tail. Begin video recording immediately after suspension. Observe the animal for 6 minutes, during which periods of immobility and struggling will be recorded manually or automatically, depending on the setup.
6. Post-Session Cleaning and Data Management: After the session, gently remove the tape and return the animal to its home cage. Clean the suspension area and hook again with 75% ethanol. Save and organize all video files and tracking data. Ensure proper labeling of experimental groups for subsequent analysis.
主要功能及特色
The tail suspension test apparatus is designed to evaluate behavioral despair in mice and is widely used as a standard model for screening antidepressant efficacy. Its primary function is to record the duration of immobility and active struggling when the mouse is suspended by the tail, providing a quantitative measure of stress-coping behavior. Equipped with high-resolution video recording and automated analysis software, the system accurately distinguishes between immobility and movement, reducing observer bias. Standardized tail suspension fixtures ensure consistent positioning across subjects, and some models support high-throughput testing with multiple simultaneous channels. This apparatus is commonly applied in neuropsychiatric research, particularly for assessing depression-like behaviors, validating antidepressant compounds, and studying emotional phenotypes in genetic or disease models.
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