爬杆实验 Pole-climbing test

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设备型号

SA111

当前状态

管理员

李存正 Cunzheng LI 020-88338688

放置地点

E4-4F LAF DEF侧入口-432A一更-432B二更-E区433A-1行为学E1433A-2行为学E2
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名称

爬杆实验 Pole-climbing test

资产编号

LAF202501061

型号

SA111

规格

产地

中国

厂家

赛昂斯

所属品牌

赛昂斯

出产日期

2024-12-22

购买日期

2024-12-22

所属单位

实验动物中央实验室 Laboratory Animal Facility (GZ)

使用性质

科研

所属分类

LAF-行为测试 Behavioural test devices

资产负责人

李存正 Cunzheng LI

联系电话

020-88338688

联系邮箱

放置地点

E4-4F LAF DEF侧入口-432A一更-432B二更-E区433A-1行为学E1433A-2行为学E2
  • 主要规格&技术指标
  • 主要功能及特色
主要规格&技术指标
爬杆材质:松香木;
爬杆尺寸:圆球直径 2.5cm,杆长 50cm,杆直径 1cm,爬杆与底板倾斜角度可任意调节;

1. Pre-Test Preparation: Ensure all equipment is working properly, including the pole, stopwatch (or camera), and data sheets. Maintain a quiet environment with consistent lighting. Allow mice to acclimate in the testing room for at least 30 minutes before starting.
2. Apparatus Setup and Cleaning: Use a rosin pine pole with a 2.5 cm diameter top ball, 50 cm pole length, and 1 cm diameter. The pole angle can be freely adjusted as needed. Clean the pole and baseboard with 75% ethanol before each trial and let dry completely.
3. Behavioral Testing Procedure: Place the mouse head-upward on the top of the ball. Start timing immediately. Record the time to turn downward (T-turn) and the total time to reach the base (T-total). If the mouse does not turn in 30 seconds or reach the base in 60 seconds, record as a failed trial.
4. Trial Repeats: Each mouse performs 3 trials with 2–3 minutes of rest in between. Use the average of T-turn and T-total for analysis.
5. Post-Session Cleaning and Data Management: Clean the apparatus with 75% ethanol after each session. Return the mouse to its home cage and record all relevant data.
Note: The protocol can be adjusted according to specific experimental designs, such as altering the pole angle, trial number, or testing intervals.
主要功能及特色
The pole test is a simple and effective assay used to evaluate motor coordination, balance, and bradykinesia in rodents. It is widely applied in Parkinson’s disease models and neurodegenerative research. Featuring a rough-surfaced pole and adjustable incline, the test offers high sensitivity and reproducibility for assessing motor deficits and drug effects.
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