大组织样本光片显微成像系统 Large sample Light-sheet Microscope
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7/人使用者
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102/次机时次数
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1767.39/小时总时长
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0/次送样次数
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5/人收藏者
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收费标准
机时0元/小时 -
设备型号
LiTone XL -
当前状态
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管理员
李思仪 Rita Siyi LI,刘健威 Jianwei LIU -
放置地点
E4-123FG
- 仪器信息
- 预约资源
- 附件下载
- 公告
- 同类仪器
名称
大组织样本光片显微成像系统 Large sample Light-sheet Microscope
资产编号
BC-MS-0023
型号
LiTone XL
规格
产地
厂家
所属品牌
LiT
出产日期
购买日期
所属单位
生物科学中央实验室 Biosciences Central Research Facility (GZ)
使用性质
科研
所属分类
BioCRF-影像平台 Imaging Core
资产负责人
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联系电话
联系邮箱
ritasiyili@hkust-gz.edu.cn
放置地点
E4-123FG
- 主要规格&技术指标
- 主要功能及特色
- 设备使用相关说明
主要规格&技术指标
1. Lasers: 405nm (DAPI), 488nm (GFP), 561nm (mCherry), 647nm (Alexa647)
2. Available objectives:
* 4x/0.28 Air lens, WD 28.8-30.0mm
* 10x/0.6, adaptable RI 1.33-1.52, WD 8mm
* 25x/1.0, adaptable RI 1.41-1.52, WD 8mm
* 16x/0.8 Water lens, WD 3mm
3. Field of View：3x3mm/1.2x1.2mm FOV for 4X/10X objectives. Can be multiplied by stitching
4. Illumination Mode：Four-side/two-side/single-side illumination with Line Bessel Sheet (LBS) technology
5.Light-sheet Parameter Settings
Mode 1: Thickness=1μm; For 20X objective
Mode 2: Thickness=1.5μm; For 10X objective
Mode 3: Thickness=3μm; For 4X objective
6. Sample Size: From 200μm to 20 mm diameter
7. Detection Camera sCMOS camera, 16bits, 2048 by 2048 pixels full frame, 6.5μm pixel size; Peak Quantum Efficiency > 82% @ 560nm
8. Detection Speed: 80 frame per second at 2048 x 2048 frame size
9. Spatial Resolution: For 10X air objective: 0.6μm lateral by 1.5 μm axial (with light-sheet mode 2)
10. Sample Mounting: Transparent-well mounting for cleared tissue samples; FEP or agarose mounting for live or fixed embryos
11. Sample Positioning: Full motorized high-precision XYZ sample stages
12. Software: LitScan microscope control & data rendering software. Real time 3D rendering, auto stitching & deconvolution included.
2. Available objectives:
* 4x/0.28 Air lens, WD 28.8-30.0mm
* 10x/0.6, adaptable RI 1.33-1.52, WD 8mm
* 25x/1.0, adaptable RI 1.41-1.52, WD 8mm
* 16x/0.8 Water lens, WD 3mm
3. Field of View：3x3mm/1.2x1.2mm FOV for 4X/10X objectives. Can be multiplied by stitching
4. Illumination Mode：Four-side/two-side/single-side illumination with Line Bessel Sheet (LBS) technology
5.Light-sheet Parameter Settings
Mode 1: Thickness=1μm; For 20X objective
Mode 2: Thickness=1.5μm; For 10X objective
Mode 3: Thickness=3μm; For 4X objective
6. Sample Size: From 200μm to 20 mm diameter
7. Detection Camera sCMOS camera, 16bits, 2048 by 2048 pixels full frame, 6.5μm pixel size; Peak Quantum Efficiency > 82% @ 560nm
8. Detection Speed: 80 frame per second at 2048 x 2048 frame size
9. Spatial Resolution: For 10X air objective: 0.6μm lateral by 1.5 μm axial (with light-sheet mode 2)
10. Sample Mounting: Transparent-well mounting for cleared tissue samples; FEP or agarose mounting for live or fixed embryos
11. Sample Positioning: Full motorized high-precision XYZ sample stages
12. Software: LitScan microscope control & data rendering software. Real time 3D rendering, auto stitching & deconvolution included.
主要功能及特色
LiTone XL is designed for large sample as big as 20mm and is perfect for cleared tissue, with live imaging capability and balanced resolution and FOV, providing 500nm lateral resolution and 1.5μm axial resolution over the entire sample.
LiTone XL features the same LBS technology as in LiTone LBS systems but reconfigured to provide a much larger field of view for imaging large for tissue-level imaging. Besides, XL applies a unique 4-side illumination to achieve better sample penetration and uniform capture over the entire sample.
LiTone XL is designed with optics to be adaptable to a wide range of refractive indices, from water to solvent solutions with IR as high as 1.54, which allows users to image both cleared tissue and live embryos without changing any optics manually.
Equipped with LitScan 2.0, the system also provides powerful on-the-fly 3D rendering package.
LiTone XL features the same LBS technology as in LiTone LBS systems but reconfigured to provide a much larger field of view for imaging large for tissue-level imaging. Besides, XL applies a unique 4-side illumination to achieve better sample penetration and uniform capture over the entire sample.
LiTone XL is designed with optics to be adaptable to a wide range of refractive indices, from water to solvent solutions with IR as high as 1.54, which allows users to image both cleared tissue and live embryos without changing any optics manually.
Equipped with LitScan 2.0, the system also provides powerful on-the-fly 3D rendering package.
设备使用相关说明
凡涉及安全风险的样品（如生物危害、危险化学品、人体来源样品、纳米材料、放射性材料等）须通过生物科学中央实验室准入审查；未通过审查不得入内。相关合规义务及由此产生的责任由本人及所属机构自行承担。
Any samples involving safety risks (such as biohazards, hazardous chemicals, human-derived samples, nanomaterials, or radioactive materials) must pass the BioCRF's access review; samples that do not pass the review may not be brought into the BioCRF. Compliance obligations and any resulting liabilities shall be borne by me and my affiliated institution.
Any samples involving safety risks (such as biohazards, hazardous chemicals, human-derived samples, nanomaterials, or radioactive materials) must pass the BioCRF's access review; samples that do not pass the review may not be brought into the BioCRF. Compliance obligations and any resulting liabilities shall be borne by me and my affiliated institution.
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