LC-MS system (Orbitrap Exploris 480-Vanquish Neo & Vanquish Flex)
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2/人使用者
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23/次总次数
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2100/小时总时长
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4/人收藏者
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收费标准
机时0元/小时送样详见检测项目 -
设备型号
Orbitrap Exploris 480+Vanquish Flex&Neo -
当前状态
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管理员
文美玲 Wendy Meiling WEN 020-88338595 -
放置地点
E4-126/133走廊-132A
- 仪器信息
- 检测项目
- 附件下载
- 公告
- 同类仪器
名称
LC-MS system (Orbitrap Exploris 480-Vanquish Neo & Vanquish Flex)
资产编号
BC-PM-0007
型号
Orbitrap Exploris 480+Vanquish Flex&Neo
规格
产地
厂家
所属品牌
Thermo Fisher Scientific
出产日期
购买日期
所属单位
生物科学中央实验室 Biosciences Central Research Facility (GZ)
使用性质
科研
所属分类
BioCRF-蛋白质&代谢组学平台 Proteomics & Metabolomics Core
资产负责人
文美玲 Wendy Meiling WEN
联系电话
020-88338595
联系邮箱
meilingwen@hkust-gz.edu.cn
放置地点
E4-126/133走廊-132A
- 主要规格&技术指标
- 主要功能及特色
- 样本检测注意事项
- 设备使用相关说明
- 备注
主要规格&技术指标
Scan rate Up to 40 Hz at resolution setting 7500 at m/z 200
Multiplexity: up to 20 precursors/scan
Resolution Up to 480,000 at m/z200
Mass accuracy < 3 ppm RMS drift over 24 hours using external calibratior
< 1 ppm RMS drift over 24 hours using internal calibration
Sensitivity Dynamic range > 5000 within a single Orbitrap mass analyzer spectrum
MS/MS: 50 fg reserpine on column S/N 100:1
SlM: 50 fg reserpine on column S/N 150:1
Features Higher Energy Collisional Dissociation (HCD)
EASY-ICTM (internal calibration) source
Quadrupole mass flter
lon Routing Multipole (lRM) trapping, focuses and routes ions more effectively
Orbitrap mass analyzer
Broader range of experiments with multiple scan modes: data-dependent acquisition (DDA), data independent acquisition (DlA), spectral multiplexing (lMSx, TopN and new TopSpeed (TopS), BoxCar DlA and DDA experiments, tSlM, tSlM MSx, ddMS/MS, targeted MS/MS
Multiplexity: up to 20 precursors/scan
Resolution Up to 480,000 at m/z200
Mass accuracy < 3 ppm RMS drift over 24 hours using external calibratior
< 1 ppm RMS drift over 24 hours using internal calibration
Sensitivity Dynamic range > 5000 within a single Orbitrap mass analyzer spectrum
MS/MS: 50 fg reserpine on column S/N 100:1
SlM: 50 fg reserpine on column S/N 150:1
Features Higher Energy Collisional Dissociation (HCD)
EASY-ICTM (internal calibration) source
Quadrupole mass flter
lon Routing Multipole (lRM) trapping, focuses and routes ions more effectively
Orbitrap mass analyzer
Broader range of experiments with multiple scan modes: data-dependent acquisition (DDA), data independent acquisition (DlA), spectral multiplexing (lMSx, TopN and new TopSpeed (TopS), BoxCar DlA and DDA experiments, tSlM, tSlM MSx, ddMS/MS, targeted MS/MS
主要功能及特色
Orbitrap Exploris 480质谱仪是革命性的新一代模块化超高分辨率质谱仪，它在保持高分辨率、高质量精度和图像质量的同时占用更少的空间。它结合了成熟的技术、先进的功能和智能驱动的分析框架，使研究者能够更广泛地使用质谱法进行高通量蛋白质鉴定、定量和结构表征。
Vanquish Flex UHPLC系统有三种泵类型，可满足吞吐量、灵活性或先进的工作流程要求。它应用了五种替代检测原理，并能够与赛默飞科学质谱仪无缝集成。
Vanquish™Neo UHPLC系统为致力取得科学新突破的研究人员提供了全天候 (24/7) 卓越性能的纳流、毛细管流和微流 LC-MS。从组学和生物制药到高通量转化研究，Vanquish Neo UHPLC系统是低流LC 的新标准。
The Orbitrap Exploris 480 mass spectrometer is a revolutionary new generation of modular ultra high-resolution mass spectrometry (ums) that takes up less space while maintaining high-resolution, high-quality accuracy and image quality. It combines mature technologies, advanced functions, and an intelligent driven analytical framework for the wider use of mass spectrometry by research scientists for high-throughput protein identification, quantitative and structural characterization.
Vanquish Flex UHPLC System has three pump types to meet throughput, flexibility, or advanced workflow requirements. It applies five alternative detection principles and enables seamless integration with the Thermo Scientific mass spectrometer.
Vanquish™ Neo UHPLC system delivers maximum performance 24/7 for nano-, capillary-, and micro-flow LC-MS. From -omics and biopharma to high-throughput translational research, the Vanquish Neo UHPLC system is the new standard in low-flow LC.
Vanquish Flex UHPLC系统有三种泵类型，可满足吞吐量、灵活性或先进的工作流程要求。它应用了五种替代检测原理，并能够与赛默飞科学质谱仪无缝集成。
Vanquish™Neo UHPLC系统为致力取得科学新突破的研究人员提供了全天候 (24/7) 卓越性能的纳流、毛细管流和微流 LC-MS。从组学和生物制药到高通量转化研究，Vanquish Neo UHPLC系统是低流LC 的新标准。
The Orbitrap Exploris 480 mass spectrometer is a revolutionary new generation of modular ultra high-resolution mass spectrometry (ums) that takes up less space while maintaining high-resolution, high-quality accuracy and image quality. It combines mature technologies, advanced functions, and an intelligent driven analytical framework for the wider use of mass spectrometry by research scientists for high-throughput protein identification, quantitative and structural characterization.
Vanquish Flex UHPLC System has three pump types to meet throughput, flexibility, or advanced workflow requirements. It applies five alternative detection principles and enables seamless integration with the Thermo Scientific mass spectrometer.
Vanquish™ Neo UHPLC system delivers maximum performance 24/7 for nano-, capillary-, and micro-flow LC-MS. From -omics and biopharma to high-throughput translational research, the Vanquish Neo UHPLC system is the new standard in low-flow LC.
样本检测注意事项
1. 蛋白组学
若需蛋白前处理服务，具体方式请咨询仪器管理员；
若送肽段样品，请用户自行脱盐定量后送样；
不提供肽段标记服务，若用户需做标记蛋白组学，须自行标记肽段后再送样；
蛋白组学样本信息收集表详见附件”Proteomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
2. 小分子直接MS进样
需提供样品纯度证明，如：NMR谱图，LC-UV色谱图或者低分辨质谱图；
样本信息收集表详见附件”Small Molecule MS_Sample Submission Form“，送样时填写并交给仪器管理员；
3. 代谢组学
不提供样品前处理服务，用户需自行处理样品，并制备QC样品；
样本信息收集表详见附件”Metabolomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
4. 每周一和周三接收样品（E4-122）；
5. 如有不明确的其他事宜，请联系仪器管理员。
若需蛋白前处理服务，具体方式请咨询仪器管理员；
若送肽段样品，请用户自行脱盐定量后送样；
不提供肽段标记服务，若用户需做标记蛋白组学，须自行标记肽段后再送样；
蛋白组学样本信息收集表详见附件”Proteomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
2. 小分子直接MS进样
需提供样品纯度证明，如：NMR谱图，LC-UV色谱图或者低分辨质谱图；
样本信息收集表详见附件”Small Molecule MS_Sample Submission Form“，送样时填写并交给仪器管理员；
3. 代谢组学
不提供样品前处理服务，用户需自行处理样品，并制备QC样品；
样本信息收集表详见附件”Metabolomics MS_Sample Submission Form“，送样时填写并交给仪器管理员；
4. 每周一和周三接收样品（E4-122）；
5. 如有不明确的其他事宜，请联系仪器管理员。
设备使用相关说明
蛋白组学分析默认1小时梯度，延长梯度加收20元/小时。
备注
1. Shotgun Protein Identification
• In-solution tryptic digestion service available
• Database search using Proteome Discoverer software (DDA) or PEAKS Studio (DDA & DIA)
2. Protein Quantification
• Label-free Quantification (DDA or DIA)
• Labeling Quantification by MS2 (up to 18-plex for TMT labels)
3. Direct infusion MS analysis for high purity sample (small molecule)
4. Untargeted Metabolomics Analysis (single mode)
• In-solution tryptic digestion service available
• Database search using Proteome Discoverer software (DDA) or PEAKS Studio (DDA & DIA)
2. Protein Quantification
• Label-free Quantification (DDA or DIA)
• Labeling Quantification by MS2 (up to 18-plex for TMT labels)
3. Direct infusion MS analysis for high purity sample (small molecule)
4. Untargeted Metabolomics Analysis (single mode)
检测项目
附件下载
公告
同类仪器
