T迷宫（小鼠）
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2/人使用者
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8/次机时次数
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39/小时总时长
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0/次送样次数
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0/人收藏者
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收费标准
机时0元/小时 -
设备型号
SA205M -
当前状态
-
管理员
李存正 Cunzheng LI 020-88335662 -
放置地点
E4-1F-137C清洗消毒室138A洁物存储间137A一更138F检疫室
- 仪器信息
- 预约资源
- 附件下载
- 公告
- 同类仪器
名称
T迷宫（小鼠）
资产编号
LAF202501031
型号
SA205M
规格
SA205M
产地
中国
厂家
赛昂斯
所属品牌
赛昂斯
出产日期
2024-12-22
购买日期
2024-12-22
所属单位
实验动物中央实验室 Laboratory Animal Facility (GZ)
使用性质
科研
所属分类
LAF-行为测试 Behavioural test devices
资产负责人
李存正 Cunzheng LI
联系电话
020-88335662
联系邮箱
放置地点
E4-1F-137C清洗消毒室138A洁物存储间137A一更138F检疫室
- 主要规格&技术指标
- 主要功能及特色
主要规格&技术指标
小鼠尺寸：起始臂长：350mm，目标臂长 280mm,宽50mm，高 200mm；
SOP:
1. Pre-Test Preparation: Ensure that all equipment, including the video camera, data acquisition system, and behavioral tracking software, is functioning properly. Turn on the room lights and maintain consistent ambient lighting to minimize variability. Animals should be transferred to the testing room in their home cages and allowed to acclimate for at least 30 minutes before testing.
2. Maze Cleaning: Before each trial, thoroughly clean the entire T-maze—including the start arm, choice arms, and walls—using 75% ethanol to remove any residual odors or biological traces from previous animals. Allow all surfaces to air dry completely before introducing a new animal.
3. Camera and Mounting Setup: Connect the camera to the tracking system via a video capture card. Adjust the camera height and angle to ensure that the full T-maze is clearly visible in the video feed. Ensure that the maze is centered and not distorted in the software interface, and that the camera is focused and level.
4. EthoVision XT Software Setup: Launch the EthoVision XT software and create a new project or load an existing T-maze configuration. Define the start arm and both goal arms as individual zones. Verify zone positions using the live camera feed to ensure alignment with the physical maze. Configure detection settings for accurate animal tracking, and set the trial duration (commonly 5–10 minutes per animal).
5. Behavioral Testing Procedure: Gently place the animal at the start arm of the T-maze, facing the intersection. Avoid sudden movements or noises. Allow the animal to explore and make a choice between the two arms. Depending on the specific protocol (e.g., spontaneous alternation, reward-based task), follow appropriate trial logic and timing.
6. Post-Session Cleaning and Data Management: After each trial, stop the recording and return the animal to its home cage. Immediately clean the T-maze again with 75% ethanol to remove any waste or odor cues. Save and back up the tracking data, and prepare the maze for the next subject.
SOP:
1. Pre-Test Preparation: Ensure that all equipment, including the video camera, data acquisition system, and behavioral tracking software, is functioning properly. Turn on the room lights and maintain consistent ambient lighting to minimize variability. Animals should be transferred to the testing room in their home cages and allowed to acclimate for at least 30 minutes before testing.
2. Maze Cleaning: Before each trial, thoroughly clean the entire T-maze—including the start arm, choice arms, and walls—using 75% ethanol to remove any residual odors or biological traces from previous animals. Allow all surfaces to air dry completely before introducing a new animal.
3. Camera and Mounting Setup: Connect the camera to the tracking system via a video capture card. Adjust the camera height and angle to ensure that the full T-maze is clearly visible in the video feed. Ensure that the maze is centered and not distorted in the software interface, and that the camera is focused and level.
4. EthoVision XT Software Setup: Launch the EthoVision XT software and create a new project or load an existing T-maze configuration. Define the start arm and both goal arms as individual zones. Verify zone positions using the live camera feed to ensure alignment with the physical maze. Configure detection settings for accurate animal tracking, and set the trial duration (commonly 5–10 minutes per animal).
5. Behavioral Testing Procedure: Gently place the animal at the start arm of the T-maze, facing the intersection. Avoid sudden movements or noises. Allow the animal to explore and make a choice between the two arms. Depending on the specific protocol (e.g., spontaneous alternation, reward-based task), follow appropriate trial logic and timing.
6. Post-Session Cleaning and Data Management: After each trial, stop the recording and return the animal to its home cage. Immediately clean the T-maze again with 75% ethanol to remove any waste or odor cues. Save and back up the tracking data, and prepare the maze for the next subject.
主要功能及特色
The T-maze is a classic behavioral apparatus primarily used to assess spatial learning, working memory, cognitive flexibility, and reward-based decision-making in rodents. It consists of a simple T-shaped structure with one start arm and two choice arms. By training animals to make specific choices between the arms, researchers can investigate their ability to learn spatial cues, update memory, or form stimulus-response associations. The T-maze is widely applied in neuroscience, pharmacology, and behavioral genetics, and is especially useful for studying cognitive deficits related to hippocampal function, prefrontal cortex regulation, Alzheimer’s disease, depression, and anxiety models.
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